cy3 rabbit anti goat igm  (Bioss)

Journal: Neural Regeneration Research

Article Title: miRNA-21-5p is an important contributor to the promotion of injured peripheral nerve regeneration using hypoxia-pretreated bone marrow–derived neural crest cells

doi: 10.4103/1673-5374.390956

Figure Lengend Snippet: The application information of antibodies

Article Snippet: Goat anti-rabbit-IgM-Cy3 , 1:200 , Abcam , Cambridge, UK , ab6939 , AB_955021.

Techniques:

Journal: Neural Regeneration Research

Article Title: miRNA-21-5p is an important contributor to the promotion of injured peripheral nerve regeneration using hypoxia-pretreated bone marrow–derived neural crest cells

doi: 10.4103/1673-5374.390956

Figure Lengend Snippet: Characterization of BM-NCCs and BM-MSCs. (A) BM-NCCs formed spherical colonies in non-adherent culture. (B) Adherently cultured BM-NCCs were round or a short spindle shape. (C) Adherent BM-MSCs had a fibroblast-like shape. (D, E) Floating BM-NCC spheres positively expressed nestin (red, Cy3), CD29 (green, FITC), CD133 (red, Cy3), and vimentin (green, FITC) with DAPI (blue) labeled nuclei. (F) Adherent BM-NCCs positively expressed nestin, CD29 (green, FITC), CD133 (red, Cy3), and vimentin (green, FITC) with proliferation marker, Ki67 (red, Cy3), and DAPI (blue) labeled cell nuclei. (G) BM-MSCs expressed stem cell marker CD90 (green, FITC) with nestin and vimentin, and lower expression of CD133 (red, Cy3); cell nuclei were labeled with DAPI (blue). Scale bars: 50 μm. BM-MSCs: Bone marrow mesenchymal stem cells; BM-NCCs: bone marrow neural crest cells; DAPI: diaminophenyl indole; FITC: fluorescein isothiocyanate.

Article Snippet: Goat anti-rabbit-IgM-Cy3 , 1:200 , Abcam , Cambridge, UK , ab6939 , AB_955021.

Techniques: Cell Culture, Labeling, Marker, Expressing

Journal: Neural Regeneration Research

Article Title: miRNA-21-5p is an important contributor to the promotion of injured peripheral nerve regeneration using hypoxia-pretreated bone marrow–derived neural crest cells

doi: 10.4103/1673-5374.390956

Figure Lengend Snippet: Promotion of axonal outgrowth and regrowth by miR-21-5p-enriched H-NCC-EVs and miR-21-5p on neurons. (A) Relatively higher expression of miR-21-5p in H-NCC-EV-treated neurons. (B) Relatively higher expression of miR-21-5p in neurons of the mimic transfection group. (C) Schematic of neurons cultured in a microfluidic device. Primary neurons were loaded in the soma chamber side, and axons traversed the microgrooves to extend into the axon chamber side. This was followed by TUJ1 (green, FITC) immunofluorescence staining analysis. (D, F) Representative images of TUJ1 (red, Cy3) stained axons of intact neurons at 72 hours and regenerated axons at 24 hours after treatment respectively in different groups. This showed longer axons in the miR-21-5p mimics group and H-NCC-EV group compared with those in the control group and NC group. The pro-growth or pro-regrowth effect of H-NCC-EVs could be attenuated by antagomiR-21-5p. Scale bars: 100 μm. (E) Scatter plot showing a longer average length of outgrowing axons in the H-NCC-EV and miR-21-5p groups. Outgrowth could be attenuated by antagomiR-21-5p. (G) Scatter plot showing a longer average length of re-growing axons post-axotomy in the H-NCC-EV and miR-21-5p groups; re-growth could be attenuated by antagomiR-21-5p. Data are presented as mean ± SEM ( n = 3). * P < 0.05, ** P < 0.01 (Student’s t -test for A; one-way analysis of variance and Tukey’s multiple comparison test for B, E, and G). Axotomy + EV + antagomiR-21-5p: Axotomy + H-NCC-EV + antagomiR-21-5p; EV + antagomiR-21-5p: H-NCC-EV + antagomiR-21-5p; FITC: fluorescein isothiocyanate; H-NCC-EVs: hypoxia-pretreated neural crest cell-derived extracellular vesicles; TUJ1: β-tubulin III.

Article Snippet: Goat anti-rabbit-IgM-Cy3 , 1:200 , Abcam , Cambridge, UK , ab6939 , AB_955021.

Techniques: Expressing, Transfection, Cell Culture, Immunofluorescence, Staining, Control, Comparison, Derivative Assay